rabbit anti troap antibody  (Proteintech)

Journal: CNS Neuroscience & Therapeutics

Article Title: TROAP regulates cell cycle and promotes tumor progression through Wnt/β‐Catenin signaling pathway in glioma cells

doi: 10.1111/cns.13688

Figure Lengend Snippet: GSEA enrichment analysis of TROAP using the GEO database. (A, B) KEGG pathways were screened with the Fold change value greater than 0 and p < 0.05 as screening conditions. The dotplot showed the 19 upregulated (NES > 0) and 4 downregulated (NES < 0) pathways. (C) GSEA enrichment plots represented that enrichment of Wnt/β‐Catenin pathway. (D, E) Immunofluorescence showing overexpression of TROAP increased level of Axin2 protein in the cytoplasm of TJ905 cells compared to control, meanwhile, TROAP overexpression also upregulated the accumulation of β‐Catenin in the cytoplasm and nucleus of TJ905 cells compared to control. Immunofluorescence quantification analysis was shown in E. (F, G) Immunofluorescence assay on U251 cells transfected with TROAP siRNA (* p < 0.05, ** p < 0.01, *** p < 0.001, respectively)

Article Snippet: Western blot analysis was performed by the following antibodies: TROAP rabbit mAb (Proteintech, 13634‐1‐AP, 1:2000), β‐catenin rabbit mAb (No.66379‐1‐Ig, 1:4000), MMP‐7 rabbit mAb (No.1 0374‐2‐AP, 1:3000), CDK2 rabbit mAb (No.10122‐1‐AP, 1:800), CDK4 rabbit mAb (No.11026‐1‐AP, 1:3000), Axin2 rabbit mAb(No. ET1703‐96; WB 1:3000), MMP‐2 rabbit mAb (No.10373‐2‐AP, 1:3000), RHOA rabbit mAb (No.10749–1‐AP, 1:2000), ROCK1 rabbit mAb (No.21850‐1‐AP, 1:2000), GAPDH mouse mAb(No.60004‐1‐Ig, 1:3000).

Techniques: Immunofluorescence, Over Expression, Control, Transfection

Journal: CNS Neuroscience & Therapeutics

Article Title: TROAP regulates cell cycle and promotes tumor progression through Wnt/β‐Catenin signaling pathway in glioma cells

doi: 10.1111/cns.13688

Figure Lengend Snippet: TROAP‐induced malignant phenotype via activating the Wnt/β‐Catenin signaling in glioma cells. (A, B) Western blot analysis showing the expression of Wnt/β‐Catenin signaling‐related proteins after silencing Axin2 or β‐Catenin in TJ905 cells ectopically overexpressing TROAP. The statistics were shown in the histogram C and D. The effect of TROAP overexpression on cell proliferation(E‐H), migration and invasion (I, J) was partly eliminated by silencing Axin2 or β‐Catenin (* p < 0.05, ** p < 0.01, *** p < 0.001, respectively)

Article Snippet: Western blot analysis was performed by the following antibodies: TROAP rabbit mAb (Proteintech, 13634‐1‐AP, 1:2000), β‐catenin rabbit mAb (No.66379‐1‐Ig, 1:4000), MMP‐7 rabbit mAb (No.1 0374‐2‐AP, 1:3000), CDK2 rabbit mAb (No.10122‐1‐AP, 1:800), CDK4 rabbit mAb (No.11026‐1‐AP, 1:3000), Axin2 rabbit mAb(No. ET1703‐96; WB 1:3000), MMP‐2 rabbit mAb (No.10373‐2‐AP, 1:3000), RHOA rabbit mAb (No.10749–1‐AP, 1:2000), ROCK1 rabbit mAb (No.21850‐1‐AP, 1:2000), GAPDH mouse mAb(No.60004‐1‐Ig, 1:3000).

Techniques: Western Blot, Expressing, Over Expression, Migration